A novel micronucleus in vitro assay utilizing human hematopoietic stem cells
نویسندگان
چکیده
منابع مشابه
A novel micronucleus in vitro assay utilizing human hematopoietic stem cells.
The induction of micronucleated reticulocytes in the bone marrow is a sensitive indicator of chromosomal damage. Therefore, the micronucleus assay in rodents is widely used in genotoxicity and carcinogenicity testing. A test system based on cultured human primary cells could potentially provide better prediction compared to animal tests, increasing patient safety while also implementing the 3Rs...
متن کاملIn vitro Assay of Human Gingival Scaffold in Differentiation of Rat’s Bone Marrow Mesenchymal Stem Cells to Keratinocystes
Objective(s)Tissue engineering is an attractive science because it promises new therapeutic strategies for repairing organs that have lost functions due to damage. The purpose of this study was to evaluate induction effect of human gingival scaffold in tissue engineering for skin regeneration.Materials and MethodsTissue samples were obtained from crown-lengthening procedures and wisdom teeth re...
متن کاملMicroRNA Modulation during the In vitro Culture of Hematopoietic Stem Cells Prior to Transplantation
Background: Human umbilical cord blood (HUCB) is an acceptable and readily accessible source of stem cells. There is an ongoing interest in cord blood stem cell therapies; however, little is known about the possible unfavorable effects of laboratory modifications on the isolated HUCB cells. The involvement of miRNAs in several biological processes has been shown. The aim of this study was to ev...
متن کاملIncreased mir33 Expression in Expanded Hematopoietic Stem Cells Cultured on Adipose Stem Cells Feeder layer
Bachgroun: Hematopoietic stem cell derived from umbilical cord blood (UCB) has been used for regenerative medicine in hematological abnormalities. MicroRNAs are important regulators of gene expression that control both physiological and pathological processes such as development of tissue and cancer. Some studies have shown that miR-33, has a critical role in control of self-renewal cells. He...
متن کاملdevelopment of a pcr assay to detect mycoplasma contamination in cord blood hematopoietic stem cells.
background and objectives: contamination of cell lines and biological products is one of the major problems of cell culture techniques. rapid detection of mycoplasma contamination in cell culture is an important part of quality control standards in related laboratories. the aim of this study was to evaluate the efficacy of pcr in detection of myroplasma as contaminants in cell cultures and othe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Toxicology in Vitro
سال: 2015
ISSN: 0887-2333
DOI: 10.1016/j.tiv.2015.07.018